I participated in research related to adipose-derived stem cells (ADSCs), spheroid culture, and cartilage regeneration. My role was primarily hands-on experimental support across cell and molecular biology workflows and in vivo experiments.
RESEARCH EXPERIENCE · 2023
Dongguk University.
Undergraduate research experience in cell-based regenerative medicine at Im Gun-il Lab.
Research Context
Experimental Experience
CELL-BASED WORK
ADSC & spheroid culture
Worked with adipose-derived and bone marrow-derived cell samples obtained through Dongguk University Hospital. Participated in cell culture, spheroid culture, and bone marrow preparation workflows.
MOLECULAR ANALYSIS
PCR & Western blot
Participated in PCR / RT-qPCR and Western blot experiments used to evaluate cellular and molecular changes in the research workflow.
TISSUE PROCESSING
Microtome experience
Gained hands-on experience with tissue sectioning using a microtome as part of downstream tissue analysis.
FACS Experience
I was introduced to FACS-based cellular analysis by observing and learning the workflow alongside a researcher. I did not independently perform the full analysis or interpret the final datasets, so I present this as an exposure to the experimental workflow rather than as an independent FACS analysis skill.
In Vivo Experience
- Mouse and rabbit handling and experimental preparation
- Anesthesia injection and preparation procedures
- Hair removal and animal handling
- Direct intra-articular administration
- Weekly GFP imaging to observe persistence over time
- Organization and recording of experimental observations
What I Learned
This experience gave me an early foundation in cell-based research and exposed me to the connection between in vitro experiments and in vivo evaluation. It also helped me become comfortable moving between cell culture, molecular assays, tissue processing, and animal experiments.
Selected Data
01 · RT-qPCR
Molecular expression analysis
Representative RT-qPCR results for Aggrecan, SOX9-1, OCN, HVEGF, H18S, and BSP. The figures preserve the original Prism-style analysis, including normalization, replicate values, and reported p-values where present.
02 · SPHEROID CULTURE
3D cell culture observation
Bright-field microscopy records across culture days, including 40× and 100× views and the original experimental metadata.
03 · CELL EXPANSION
Passage-dependent growth
Cell counts were recorded across sequential passages to track expansion and compare growth profiles across cell lines. The original records are shown without replotting or alteration.
04 · WESTERN BLOT
Protein-level analysis
Western blot documentation is kept as a separate original-record page. The image itself is not cropped or artificially enhanced.
Data presentation principle. Representative evidence is shown on the portfolio, while detailed records remain accessible through dedicated data pages. Experimental figures are preserved as supplied rather than being cosmetically reconstructed.